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KMID : 0903619840250020109
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Journal of the Korean Society for Horticultural Science
1984 Volume.25 No. 2 p.109 ~ p.115
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Changes of Carbohydrates , Protein , RNA , and Activity of Hydrolytic Enzymes of Jujube Seeds during Germination
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Abstract
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This experiment was designed to examine the metabolism of reserve substances and the activity of hydrolytic enzymes during seed germination of Chinese jujube (Zixyphus jujuba Miller).
The seeds germinated in 1 to 3 days after seeding, thereafter slowly until 7 days.
There were a gradual increase in reducing sugars until 7 days after seeding with a decrease in nonreducing sugars while a decrease at early stage and then an increase at late stage in starch contents.
There was a drastic decrease in globulin and glutelin within 12 hours after seeding with gradual decrease in albumin and prolamin after 24 hours. RNA contents were maintained at a constant level throughout germinating stages.
The optimal activities were measured at pH 7.0, 8.0, 3.0, 5.0, 5.0, 3.0 and 6.0 in α-amylase, β-amylase, invertase, cellulase, phosphatase, protease and RNase, respectively.
The enzyme activities were gradually increased in each enzyme except in cellulase; there was a centinuous increase in α-amylase, β-amylase and invertase activity by 7 days after seeding and RNase activity was constantly maintained at high level from 3 days after seeding. The activities of phosphatase and protease were significantly increased at early stage and then decreased at late stage.
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